Epithalon: Complete Research Guide & Chemical Profile

Epithalon (also known as Epitalon or Epithalone) is a synthetic tetrapeptide with the molecular formula C14H22N4O9 and a molecular weight of 390.35 Da. This research compound features the amino acid sequence Ala-Glu-Asp-Gly (AEDG), making it one of the shortest peptides studied in aging research.

Originally developed based on the natural peptide epithalamin, which was isolated from the pineal gland of calves, Epithalon represents a synthetic analog designed for enhanced stability and research applications. The peptide maintains a purity standard of ≥98% in research-grade preparations, ensuring consistent results in laboratory studies.

Research interest in Epithalon centers primarily on its potential effects on telomerase activity and cellular aging processes. Laboratory studies have investigated this tetrapeptide's interaction with various biological pathways, particularly those related to circadian rhythm regulation and cellular longevity mechanisms.

Epithalon research traces back to the work of Professor Vladimir Khavinson and his colleagues at the St. Petersburg Institute of Bioregulation and Gerontology. Initial studies in the 1990s focused on understanding the peptide's effects on pineal gland function and melatonin production in animal models.

Laboratory research has demonstrated Epithalon's ability to influence telomerase activity in various cell cultures. Studies published in the Bulletin of Experimental Biology and Medicine have shown that the peptide can activate telomerase in human somatic cells, potentially affecting cellular replication limits.

Research conducted on animal models has indicated that Epithalon administration may influence circadian rhythm patterns and sleep-wake cycles. These studies suggest the peptide's interaction with pineal gland function, though the exact mechanisms require further investigation.

Long-term studies in laboratory settings have explored Epithalon's effects on various biomarkers associated with aging processes. Research indicates potential influences on antioxidant enzyme activity, though results vary depending on experimental conditions and model systems used.

Research suggests that Epithalon's primary mechanism involves the activation of telomerase, an enzyme responsible for maintaining telomere length in cells. Laboratory studies indicate that the peptide may interact with specific cellular pathways that regulate telomerase expression and activity.

The tetrapeptide appears to influence gene expression patterns related to cellular aging and stress response pathways. In vitro studies have shown that Epithalon treatment can affect the expression of genes involved in DNA repair mechanisms and cellular protection against oxidative stress.

Research indicates that Epithalon may also interact with the hypothalamic-pituitary axis, potentially influencing the production of various hormones including melatonin. This interaction could explain the peptide's observed effects on circadian rhythm regulation in laboratory models.

Studies suggest that the peptide's mechanism may involve the modulation of specific transcription factors that control cellular senescence pathways. However, the complete molecular pathway remains an active area of research investigation.

Epithalon presents as a white to off-white lyophilized powder with high water solubility. The peptide maintains stability under proper storage conditions, making it suitable for various research applications and experimental protocols.

• Molecular Weight: 390.35 Da
• Amino Acid Sequence: Ala-Glu-Asp-Gly
• Purity: ≥98% (HPLC verified)
• Solubility: Highly soluble in water and physiological buffers
• pH Stability: Stable in neutral to slightly acidic conditions

The peptide's small molecular size and hydrophilic nature contribute to its research utility in cell culture applications. Laboratory studies indicate good bioavailability in various experimental models, though stability can be affected by extreme pH conditions and prolonged exposure to light.

Research-grade Epithalon requires specific storage conditions to maintain peptide integrity and biological activity. Lyophilized powder should be stored at -20°C or below, protected from light and moisture to prevent degradation.

Once reconstituted, aqueous solutions of Epithalon should be used promptly or stored at 4°C for short-term use (up to one week). For longer storage periods, aliquoting and freezing at -80°C is recommended to maintain peptide stability and research reliability.

Researchers seeking high-quality Epithalon for laboratory studies can find verified vendors and third-party tested batches through ChemVerify's comprehensive database. Our platform provides detailed verification information, including purity certificates, analytical data, and vendor reliability ratings.

ChemVerify's verification process includes independent third-party testing for peptide purity, identity confirmation through mass spectrometry, and contamination screening. This ensures researchers receive authentic Epithalon that meets specified quality standards for reliable experimental results.

Visit ChemVerify at /product/epithalon to access our verified vendor network, compare analytical certificates, and find the most suitable Epithalon source for your research requirements. Our database includes both domestic and international suppliers with verified track records in peptide research supply.

<strong>What is the difference between Epithalon and Epitalon?</strong>

Epithalon and Epitalon refer to the same synthetic tetrapeptide compound. The spelling variation (with or without the 'h') occurs in different research publications and supplier catalogs, but both terms describe the identical Ala-Glu-Asp-Gly peptide sequence.

<strong>How should Epithalon be reconstituted for research use?</strong>

Research protocols typically reconstitute lyophilized Epithalon using sterile water or appropriate buffer solutions. The peptide's high water solubility allows for easy reconstitution, though researchers should follow specific protocols based on their experimental requirements and analytical methods.

<strong>What analytical methods are used to verify Epithalon purity?</strong>

High-Performance Liquid Chromatography (HPLC) serves as the primary method for Epithalon purity verification. Mass spectrometry (MS) provides molecular weight confirmation, while amino acid analysis can verify sequence integrity. These combined analytical approaches ensure research-grade quality.

<strong>What are the typical research applications for Epithalon?</strong>

Laboratory research applications include telomerase activity studies, cellular aging investigations, circadian rhythm research, and pineal gland function studies. Epithalon is commonly used in cell culture experiments, animal model studies, and molecular biology research focusing on aging mechanisms.

<strong>How stable is Epithalon in solution?</strong>

Research indicates that Epithalon solutions maintain stability for approximately one week when stored at 4°C. For longer-term storage, freezing at -80°C is recommended. The peptide's stability can be affected by pH extremes, temperature fluctuations, and exposure to light.

<strong>What is the relationship between Epithalon and epithalamin?</strong>

Epithalon is a synthetic analog of epithalamin, a natural peptide originally isolated from the pineal gland. While epithalamin is the naturally occurring compound, Epithalon was developed to provide enhanced stability and consistency for research applications, maintaining the same amino acid sequence and biological activity profile.

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